The numerical relationship between the number of Sertoli cells and the number of spermatozoa and all types of germ cells (collectively referred to as reproductive cells; RCs) present at any given time within the seminiferous tubules has not been well documented. The study design was to correlate the counts of various RCs, Sertoli cells, and spermatozoa in positive and negative specimens of micro TESA (mTESE) and TESE. The sample size was n=21 pts for mTESE for negative (late maturation arrest); n=14 mTESE cases positive for spermatozoa; n=19 TESE cases were negative for spermatozoa, and n=19 cases positive for spermatozoa. There were significant (p<0.00001) positive (Chi-square) interactions between various RCs in mTESE and TESE specimens, both positive and negative for spermatozoa. In the mTESE positive group, spermatozoa were significantly correlated with RCs and Sertoli cells, and Sertoli cells were significantly correlated with all the RCs (p<0.0001) and elongated spermatids (p=0.0224). In the mTESE negative group, spermatozoa were significantly correlated with Sertoli cells and primary (p=0.0118), and secondary spermatocytes, round and elongated spermatids (p<0.0001), but not with spermatogonia. In the TESE-positive group, spermatozoa were significantly correlated with Sertoli cells (p=0.0101) and round spermatids (p<0.0001) but not with other cell types (p>0.05), while Sertoli cells were significantly correlated with round spermatids (p=0.0031) but not with other cell types. In the TESE group negative for spermatozoa, there were no correlations whatsoever between all cell types (p>0.05). The proportions of spermatozoa and all cell types in the mTESE positive group were significantly different to all cell types (p<0.0001) in the corresponding negative group except for primary and secondary spermatocytes (p>0.05), but in TESE tissues, all cells were significantly (p<0.0001) different between the groups positive and negative for spermatozoa. A positive numerically interactive relationship exists between cells in the testicular specimen, which is a novel finding.